How Does Frog Survive After Being Frozen by Liquid Nitrogen?

Question by C.R.: How does frog survive after being frozen by liquid nitrogen?

Best answer:

Answer by Harish
For the wood frog (Rana sylvatica), in the winter, as much as 45% of its body may freeze and turn to ice. “Ice crystals form beneath the skin and become interspersed among the body’s skeletal muscles. During the freeze the frog’s breathing, blood flow, and heart beat cease. Freezing is made possible by specialized proteins and glucose, which prevent intracellular freezing and dehydration.” [3][4] The wood frog can survive up to 11 days frozen at -4 C.

Other vertebrates that survive at body temperatures below 0 C include painted turtles (Chrysemys picta), Gray tree frog (Hyla versicolor), Box turtles (Terrapene carolina)- 48 hours at -2 C, Spring peeper (Pseudacris crucifer), Garter snakes (Thamnophis sirtalis)- 24 hours at -1.5 C, the chorus frog (Pseudacris triseriata), Siberian salamander (Salamandrella keyserlingii), 24 hours at -15.3 C [5], Antarctic fish such as Pagothenia borchgrevinki and the European common lizard (Lacerta vivipara). [6] [7]
Professor Joshua Barr of the Staines Cyrobiology Laboratory in Middlesex (UK) has been carrying out experiments with British tree frogs to discover whether they also exhibit tolerance to very low temperatures in the same way as exhibited by American Tree Frogs. [8] Thus far results have been inconclusive, however Professor Barr is optimistic about the future. [ http://www.stainesnews.co.uk/ ]
Hibernating Arctic ground squirrels may have abdominal temperatures as low as −2.9 °C (27 °F), maintaining sub-zero abdominal temperatures for more than three weeks at a time, although the temperatures at the head and neck remain at 0 C or above.[3]
Human embryos can survive cryopreservation at -196 C for 10 years under well-controlled laboratory conditions. [9]
Some experiments have been done with living humans. See section below on cryobiology in humans.

Give your answer to this question below!

CRYSTAL ICE – Footage taken from Crystal Ice’s “Prodigy 2” performance that took place in the Tain Theatre, Dundalk on the 25th/26th August. Music: “Heads will Roll” by the Yeah Yeah Yeahs. Edited by: Tim Shearwood

The Ice Is Breaking: One Woman's Campaign to Affirm Queer Christian Youth

Filed under: crystal ice

The first thing you notice about Crystal Cheatham is her effusive energy and contagious optimism. She's on a mission. Already, at the age of 27, she is making an impact on the world through her music and her activism. Crystal is a singer/songwriter, …
Read more on Huffington Post

Icy roadway contributes to rollover accident

Filed under: crystal ice

CRYSTAL FALLS – Icy road conditions contributed to a single vehicle rollover accident that occurred Tuesday morning on U.S. 141 near the Net River in Iron County's Hematite Township. At 7:56 a.m. CST, Iron County Central Dispatch received a 911 call …
Read more on Marquette Mining Journal